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KMID : 0385220000100010035
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Korean Journal of Gerontology
2000 Volume.10 No. 1 p.35 ~ p.41
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Mitochondrial Dysfunction and Oxidative Stress in Cytotoxicity Induced by Amyloid Peptide
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Song Ji-Hyun
Park Chung-Soon
Choe Jung-Yoon
Han Bok-Ghee
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Abstract
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Recent studies suggest that oxygen free radicals are involved in the cytotoxic effects of amyloid-β-peptide (Aβ). In this study, we investigated the induction of oxidative stress and mitochondrial dysfunction in the process of cell death induced by Aβ peptides. Aβ treatment to the C6 cell increased levels of DCF-fluorescence, suggesting an increased intracellular concentration of hydrogen peroxide and furthermore an oxidative stress in cells. Activity of antioxidant enzyme catalase and superoxide dismuatase showed no significant difference between control and Aβ-treated C6 cell. The activities of mitochondrial enzymes, succinate dehydrogenase(SDH) and cytochrome C oxidase(COX) decreased by the treatment of Aβ to the C6 cells. Pretreatment of antioxidant eliminate the reductive effect of Aβ on SDH and COX activity. In the parallel experiment the Aβ-treatment also induced LDH-release, which indicates cytotoxicity. However antioxidant pretreatment did not block the reduction of LDH-release in Aβ-exposed C6 cell. liken together, our results suggest that the oxidative stress associated with mitochondrial dysfunction induced by Aβ.
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KEYWORD
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Mitochondrial dysfunction, Aβ, Oxidative Stress, C6 cell
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